Part:BBa_J100036:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J100036

This graph shows total florescence adjusted for cell density. Sample 1 was this polB promoter without any form of induction. Sample 2 was also this polB promoter, induced by adding sodium azide to cause DNA damage. Sample 3 was a negative control without a promoter. Instead it had a transcription terminator which prevented RFP from being produced. Sample 4 was a positive control in the form of constitutive promoter pTet. Sample 5 included the promoter pLacI with IPTG. Sample 6 was pLacI without IPTG. Our experimental promoter in the first tube was not induced and thus produced negligent amounts of red fluorescent protein. In contrast our induced experimental promoter produced a statistically significant amount of fluorescence.

User Reviews

UNIQ630f94f76a176f14-partinfo-00000000-QINU UNIQ630f94f76a176f14-partinfo-00000001-QINU